How Does MotifFinder work?​​

MotifFinder useses a modified regression tree algorithm to render, test, and confine binding glycans into a digestible set of motifs.​

Workflow

Can MotifFinder Be Trusted?

What Does MotifFinder Give Me?

MotifFinder​ (Glycan-Array Data Analysis Software)

Highlights of the MotifFinder Report. The full report is shown in the middle column for WGA analyzed on a glycan array. This analysis made use of data from 7 different concentrations of WGA. A) Statistics for the top motifs. B) Binding levels from a single concentration. C) Distribution of motifs ordered by glycan-binding intensity. D) Map of primary motifs providing relative binding and population size. E) Glycan binding levels of all concentrations. F) Tree relationship of primary motifs and fine specificities. G) Binding with respect to lectin concentration for each primary motif. H) Estimated binding strengths derived from the curves in panel G

References:​

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  • ​​Klamer, Z., Staal, B., Prudden, A. R., Liu, L., Smith, D. F., Boons, G. J., and Haab, B. B. (2017) Mining high-complexity motifs in glycans: A new language to uncover the fine-specificities of lectins and glycosidases. Analytical Chemistry, 89, 12342-12350.
  • Haab, B. B.; Klamer, Z. (2020) Advances in Tools to Determine the Glycan-Binding Specificities of Lectins and Antibodies. Mol Cell Proteomics, 19 (2), 224–232. https://doi.org/10.1074/mcp.R119.001836.
  • Klamer, Z., Haab, B.B (2020) Automated Identification of Lectin Fine Specificities from Glycan Array Data. Advances in Carbohydrate–Protein Interactions: Techniques and Applications. [In Press]
  • ​Resource from Dr. Brian Haab's lab.
  • Development of MotifFinder is supported by NIH SBIR program.

What Does MotifFinder Do?​​

MotifFinder automatically analyzises glycan array data to create a versitle set of motifs which deconvolves strucutres (motifs) critical for glycan binding. ​

Tools

Validation of automated analysis. A) Comparison of the canonical motif to those find in the automated MotifFinder analysis. The primary motifs and fine specificities are shown. B) Lectin binding to groups of glycans. The glycans are grouped by the fine-specificity motif they contain. Each point is a glycan, and the value is the amount of lectin binding.