Antibiotics are our most important agents for fighting bacterial infections, and the emergence of antibiotic-resistant bacteria is becoming a global health crisis that has contributed to the reemergence of diseases that were once well controlled. Antibiotics function by binding to the bacterial ribosome and inhibiting protein synthesis, but their efficacy is affected by binding to plasma proteins and antibiotic-targeting enzymes produced by bacteria. Their ribosome binding abilities also make them candidates as treatments for genetic disorders, HIV, and AIDS. The GlycoAntibiotic Array is designed to help researchers screen proteins, enzymes, or other influential agents to determine their antibiotic binding targets.
Typical Binding Assay Result from the GlycoAntibiotic Microarray
The GlycoAntibiotic Microarray was assayed with an anti-vancomycin antibody, followed by streptavidin-Cy3 conjugated anti-IgG. The array was scanned at 532nm wavelength. Binders near the bottom are positive controls and the marker, and there is strongest binding to GA-13 (Vancomycin), followed weakly by antibiotics with similar structures.