Z Biotech’s multivalent slides show greater binding affinity than planar slides: The glycan GM1 (100 μM) is printed on both a Z Biotech’s multivalent hydrazide functionalized slide and a standard planar hydrazide functionalized slide. Glycan-lectin binding is detected with biotinylated Cholera toxin B subunit (CTB-BI) and streptavidin-Cy3. Z Biotech’s multivalent slide demonstrates approximately twice the binding sensitivity (relative fluorescence units, RFU) as the planar slide.
(includes user protocols)
Z Biotech also provides optimized buffers and blockers for maintaining activity of analysis samples and obtaining high assay signal intensity for microarrays fabricated on the microarray substrates.
Shown is the surface coated side of the microarray slides. In the correct orientation, the “Z Biotech” label is located in the bottom right corner (Landscape) or bottom left corner (Portrait). Samples should be printed on the side with “Z Biotech” facing upward.
Z Biotech offers several versions of glycan, protein or DNA microarray substrates featured with arrangement of 3-dimensional and multivalent functional groups on the surface.
The NHS microarray slides contain highly reactive n-hydroxysuccinimide (NHS) ester surface, which forms covalent bonds with primary amines. The NHS slides offer high loading capacity and extremely low background for the immobilization of proteins (antibodies and antigens), amine-modified glycans, amino-modified oligonucleotides, and amine-modified small chemical molecules.
The hydrazide-functionalized substrate slides provide the only microarray slides on the market that are capable for fabricating glycan microarrays with natural glycans containing free reducing-end. Additionally, the hydrazide-functionalized slide also can be used for site-specific immobilization of proteins through the carbohydrate portion after oxidation.
Microarray slides and assay buffers
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