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Bacterial Glycan Array
Example: Bacterial Glycan Microarray on 16 subarray format. A subarray assayed with anti-LPS antibody (10 μg/ml), followed by anti-rabbit IgG, Cy3 (5 μg/ml). The array was scanned with a microarray scanner at 532nm wavelength. The positive control 4 (rabbit IgG) shows binding as expected.
Lipopolysaccharides (LPS) are the highly immunogenic endotoxins enriched on bacterial cell membranes which are the major determinants of infectious disease progression. The outer portion of the LPS - the O-antigen containing repeating glycan units - is the most variable portion of the LPS. This interactive epitope has various effects depending on the strain, often allowing the host to develop O-antigen specific protective immunity, or in some cases evolving to induce an immune response that actually helps protect the bacteria. In any case these highly involved glycan communicators are candidates for targeted antimicrobial agents or immune therapies. More research is needed to uncover the specific interactions these LPS have with the immune system in order to direct effective vaccine design.
The Bacterial Glycan Array is a research platform designed to efficiently test binding interactions with the O-antigens of bacterial LPS. Our version 1 microarray features 22 of these O-antigens purified from various bacterial strains. One microarray may be used to determine binding interactions for 8 to 16 samples each, whether the samples are sera, antibodies, or other glycan-binding proteins of interest. Shown below is an example of an anti-LPS antibody binding on the Bacterial Glycan Array.
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