Innovative Biochemical Analysis Solutions

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Example 1:  100 glycan array on 8 subarray format. A subarray assayed with glycan-binding protein biotinylated ConA lectin (10 μg/ml), followed by Streptavidin-Cy3. Array was scanned with GenePix scanner at 450 PMT and 100% laser power at 532nm wavelength.  All positive controls 1-4 and the marker show binding as expected, as well as mannose-containing glycans 60, 65, 72, 73, 98, 99, and 100.  

2. Basic Concept for Indirect Binding Assay

How Assay works

Glycan Features 

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1. Basic Concept for Direct Binding Assay

100 Glycan Array

For the 100 Glycan Array user manual, click here.

Z Biotech’s 100 Glycan Array is used as a general test to help researchers determine binding characteristics of antibodies, proteins, bacteria, cell cultures, or other potential biological samples to an array of 100 glycans representing the range of glycans found glycosylated to cell membranes. Our microarray slides are especially coated to be capable of immobilizing natural glycans with close-ring structure at their free-reducing end. 

100 Glycan Array

Example 2:  100 glycan array on 8 subarray format.  A subarray assayed with glycan-binding protein biotinylated AAL lectin (1 μg/ml), followed by Streptavidin-Cy3. Array was scanned with GenePix scanner at 500 PMT and 100% laser power at 532nm wavelength.  All positive controls 1-4 and the marker show binding as expected, as well as fucose-containing glycans 5, 26, 35, 36, 37, 38, 41, 44, 45, 46, 51, 52, 53, 54, 55, 56, 57, 64, 66, 75, 78 and 81.  

Typical Binding Assay Result from the 100 Glycan Array